Born in Bengal, Majumdar studied at the Government School of Arts and Crafts, Calcutta, where he mastered the Western Academic style of painting. This style was adapted to a predominantly Indian subject matter, especially female figures, he was also an accomplished landscape painter. Mazumdar's work may be seen in various public collections and the National Gallery of Modern Art, New Delhi.
Hemen Majumdar painted the gates to welcome King George V, on his visit to India in 1911. In 1919, he founded Indian Academy of Fine Art in Calcutta along with Jogeshchandra Seal, Jamini Roy, Bhabani Charan Law and Atul Bose. He also published a journal, Shilpi, with A.C. Mukhopadhyay.